BPC-157 — 5mg Vial

TB-500 (Thymosin Beta-4) — 5mg Vial

Mechanism of Action

BPC-157 acts via the nitric oxide (NO) system through eNOS/Src-Caveolin-1 pathway activation, upregulates VEGFR2-mediated angiogenesis, and modulates FAK-paxillin cytoskeletal signaling, driving fibroblast proliferation and ECM remodeling in experimental wound models.

TB-500 (Thymosin Beta-4) primary mechanism involves high-affinity monomeric G-actin binding (Kd ~0.5 μM), regulating the G-actin:F-actin equilibrium and cytoskeletal dynamics. By sequestering G-actin, Tβ4 modulates actin polymerization, lamellipodia formation, and directed cell migration — central to tissue repair in in vitro scratch assay models.

TB-500 also suppresses NF-κB with downstream reductions in TNF-α, IL-1β, and IL-6, activates the PI3K/Akt survival pathway, and upregulates VEGF expression in angiogenesis assays. The Ac-SDKP tetrapeptide released from the Tβ4 N-terminus is itself biologically active and studied for anti-fibrotic signaling.

Research Applications

• Dual-peptide cytoskeletal and wound-repair research protocols
• TB-500: G-actin sequestration and actin polymerization dynamics studies
• TB-500: Cell migration and lamellipodia formation assays
• TB-500: NF-κB inflammatory pathway suppression research
• TB-500: PI3K/Akt cardiomyocyte survival and cardioprotection studies
• BPC-157: VEGFR2, eNOS, and NO-pathway signaling studies

Peer-Reviewed References

1. Goldstein AL, Hannappel E, Kleinman HK. Thymosin β4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421–429.
2. Sosne G, Qiu P, Shafiee A. Thymosin β4 promotes dermal healing. Ann N Y Acad Sci. 2012;1269:68–76.
3. Bock-Marquette I, et al. Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration. Nature. 2004;432(7016):466–472.
4. Sikiric P, et al. Stable gastric pentadecapeptide BPC 157 in clinical trials. Curr Pharm Des. 2018;24(18):1942–1955.

Testing & Supply Specifications

Every Blueprint Labs batch is independently verified by third-party analytical laboratories prior to release. Identity and purity confirmed via HPLC and mass spectrometry. Full batch records maintained for chain-of-custody traceability.

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